Daurat M., Nguyen C., Dominguez Gil S., Sol V., Chaleix V., Charnay C., Raehm L., El Cheikh K., Morere A., Bernasconi M., Timpanaro A., Garcia M., Cunin F., Roessler J., Durand J.-O., Gary-Bobo M. (2023).
The mannose 6-phosphate receptor targeted with porphyrin-based periodic mesoporous organosilica nanoparticles for rhabdomyosarcoma theranostics Biomater. Sci. 8(13): 3678-3684.
Recently, we described porphyrin-based periodic mesoporous organosilica (PMO) nanoparticles synthesized from a large functional octatriethoxysilylated porphyrin precursor. The framework of the nanoparticles was formed by J-aggregates of porphyrins allowing two-photon excitation photodynamic therapy (TPE-PDT) and NIR imaging. In this study, we functionalized these PMO with polyethylene glycol (PEG) moieties and an analogue of mannose 6-phosphate functionalized on anomeric position (AMFA). These AMFA are known to efficiently target mannose 6-phosphate receptors (M6PR) which are over-expressed in various cancer cell lines (breast, prostate). Here we show that M6PR is also over-expressed in rhabdomyosarcoma (RMS) cell lines. We targeted this receptor with PMO-AMFA and efficiently performed TPE imaging and TPE-PDT of RMS cells. Furthermore the same treatment did not affect healthy myoblasts, which have a low expression of M6PR.
